Device

Part:BBa_K123009:Design

Designed by: Jason Gardiner   Group: iGEM08_University_of_Alberta   (2008-10-28)


lacIq->BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2461
    Illegal BamHI site found at 2849
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1588
    Illegal NgoMIV site found at 1986
    Illegal AgeI site found at 1365
    Illegal AgeI site found at 1477
    Illegal AgeI site found at 1906
    Illegal AgeI site found at 3258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1779
    Illegal BsaI site found at 1815
    Illegal BsaI site found at 3068


Design Notes

While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.



Source

Bisphenolics

References